csfv-specific antibodies test kit  (IDEXX)

Journal: Viruses

Article Title: Cholesterol Biosynthesis Modulates CSFV Replication

doi: 10.3390/v14071450

Figure Lengend Snippet: The alterations of intracellular cholesterol in CSFV-infected PK-15 cells. ( a ) CSFV one-step growth curve in PK-15 cells; n = 3; **** p < 0.0001. ( b ) Variation curve of intracellular cholesterol contents in CSFV-infected PK-15 cells; n = 3; *** p < 0.001; **** p < 0.0001. ( c ) Correlation analysis of CSFV replication and intracellular cholesterol levels. ( d ) The CSFV E2 protein and intracellular cholesterol levels in CSFV-infected PK-15 cells were assessed by IFA and Nile red staining. (M, mock; C, CSFV).

Article Snippet: The cells were then incubated with mouse monoclonal antibody (mAb) WH303 specific to CSFV E2 (Bioss, China) overnight at 4 °C, followed by incubation with goat anti-rabbit IgG H&L (Alexa Fluor ® 488) secondary antibody (Abcam, Boston, MA, USA) for 2 h at 37 °C, and washed with PBS three times.

Techniques: Infection, Staining

Journal: Vaccines

Article Title: Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

doi: 10.3390/vaccines9050464

Figure Lengend Snippet: Detection of CSFV RNA load in samples and tissues during 21 days after vaccination. CSFV RNA load was detected by RT-qPCR and is expressed as Ct values. (-) symbol indicates samples in which fluorescence was not detected. Grey area indicates that the tissues were not collected at that time, in accordance with the experimental design described in the Materials and Methods section.

Article Snippet: CSFV E2 specific antibodies were determined in sera samples using a commercial ELISA kit (IDEXX Laboratories, Liebfeld, Switzerland).

Techniques: Quantitative RT-PCR, Fluorescence

Journal: Vaccines

Article Title: Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

doi: 10.3390/vaccines9050464

Figure Lengend Snippet: Clinical signs monitored after challenge. The individual clinical signs were recorded after CSFV “Margarita” strain infection. Animals 2 to 7 (group A) and 14 to 18 (group C) were challenged after 21 and 5 dpv, respectively. Animals 8 to 11 (group B), contacts for group A and 19 to 23 (group D), infection control group, were not vaccinated but challenged. Pigs were monitored daily for clinical signs during the 13 dpc or until euthanasia. Different shades of colour and the numerical clinical score represent the severity of the clinical signs as shown in the legend. Super-indexed letters in the mean clinical score value from each group are employed to represent statistically significant differences between the groups on that day; similar letters indicate no statistical difference, and different letters show statistical difference ( p < 0.05).

Article Snippet: CSFV E2 specific antibodies were determined in sera samples using a commercial ELISA kit (IDEXX Laboratories, Liebfeld, Switzerland).

Techniques: Infection, Control

Journal: Vaccines

Article Title: Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

doi: 10.3390/vaccines9050464

Figure Lengend Snippet: CSFV RNA detection at different time points after challenge in samples and tissues. The samples were analyzed by RT-qPCR for ( a , c ) the CSFV and ( b , d ) Margarita strain RNA viral load at different time points during the 13 dpc (or until euthanasia). White area indicates negative samples. Euthanized animals are represented in grey square with hatches. Asterisk symbol (*) indicates statistically significant differences of the Ct values between the groups ( p < 0.05).

Article Snippet: CSFV E2 specific antibodies were determined in sera samples using a commercial ELISA kit (IDEXX Laboratories, Liebfeld, Switzerland).

Techniques: RNA Detection, Quantitative RT-PCR

Journal: Vaccines

Article Title: Early and Solid Protection Afforded by the Thiverval Vaccine Provides Novel Vaccination Alternatives Against Classical Swine Fever Virus

doi: 10.3390/vaccines9050464

Figure Lengend Snippet: IFN-α levels in sera after challenge during 13 dpc. IFN-α levels were determined at different time points after challenge with CSFV “Margarita” strain. Asterisk symbol (*) refers to 21 dpv for group A and 5 dpv for group C. Letters above the symbols are used to represent statistically significant differences between the groups on that day: similar letters indicate no statistical difference, and different letters show statistical difference ( p < 0.05).

Article Snippet: CSFV E2 specific antibodies were determined in sera samples using a commercial ELISA kit (IDEXX Laboratories, Liebfeld, Switzerland).

Techniques: